<i style="">In vitro </i>propagation of <i style="">Justicia gendarussa </i>Burm. f.–A medicinal plant

246-248An efficient protocol for in vitro propagation of Justicia gendarussa Burm. f. has been developed. MS medium supplemented with NAA induced prolific callus in both leaf and nodal explants. Organogenic and chlorophyllous calli were produced at lower concentrations of NAA (1.0 mg L‑1) and BAP (0.1 mg L-1). Thick and long roots with numerous root hairs were produced with NAA (1.0 mg L-1) and BAP (0.1 mg L-1). Long shoots were also formed. Of the in vitro grown 120 plantlets transferred to the field 94% survived after 2 months of transplantation to natural environment